Methods
Animal use and care
Female and male heterozygote non-diabetic db/+ mice
(BKS.Cg-m +/+ Leprdb/OlaHsd; Black, Lean) were purchased
from Harlan Laboratories (Mexico, DF) and were
back-crossed to start our colony. All mice had access to
water and food (Harlan Laboratories; Cat. No. T.2018S.15)
ad libitum. All animal procedures were performed in
accordance with current Mexican legislation (NOM-062-
ZOO-1999) and the National Institutes of Health (NIH,
Bethesda, MD) Guide for the Care and Use of Laboratory
Animals.