topopolymerization of diacetylene monomers in theorganized structures initiated by UV light irradiation yielding PDA that constitutes alternating double and triple bonds (ene–eyne)along its backbone [9,12]. However, for PDA/antibody vesicles thepeak intensity in 1400 cm−1and 1500 cm−1was lower than PDA vesicles. There are two hypotheses for it: first, it could indicate antibody incorporation because there was a lower peak at the sewavenumbers; and second, it could be difficult for PDA vesiclesto polymerize in antibody presence as showed in Fig. 2 [21,25].However, from colorimetric perspective, the blue color intensity was associated with the vesicle polymerization. So both vesi-cles, without and with antibody incorporation, showed the same absorbance intensity at 640 nm (blue color) indicating that the antibody was incorporated. Samples of PDA/antibody vesicles havespectra with less components in the C C and CC peaks andfewer, stronger peaks below 1400 cm−1than PDA vesicles. Thepeaks below 1400 cm−1weaken and have a more complex structurethan that of PDA/antibody vesicles [21,25].After this evidence of antibody incorporation in