Thioglycollate-induced peritonitis
Two milliliters of a sterile 4% thioglycollate solution (in phos-
phate-buffered saline) or sterile saline was injected into the
intraperitoneal cavities of C56BL/6 mice. Animals received
either a 1 mg/kg i.p. bolus of CGS21680, a 1 mg/kg i.p. bolus
of zeatin riboside or an equal volume i.p. bolus of vehicle
immediately after thioglycollate injection, 1.5 h after injection
and 3 h after injection. Mice were euthanized 5 h after initial
thioglycollateinjectionandintraperitonealcellswereharvested
via intraperitoneal wash with 6 ml cold phosphate-buffered