The pulp from two papaya fruit in each replicate was randomly sampled and homogenized at 4◦C using mortar and pestle in 10 mL of 100 mM sodium phosphate buffer, pH 6.4 containing 0.2 g of polyvinyl polypyrrolidone (PVPP) (Liu et al., 2007). The fruit pulp(2 g) was sampled from the equatorial region of the whole fruit and was a pooled sample from each replicate. The homogenates were centrifuged at 12,000 rpm for 30 min at 4◦C and the supernatant was used as the crude enzyme extract for determining the activity of polyphenol oxidase and peroxidase enzymes. The crude enzyme extracts were kept on ice during the preparation.