In vivo evaluation
Powdered roots of S. collinsiae (200 g) were weighed and exhaustively extracted with 70 % ethanol by sonication at ambient temperature. The extract was then filtered and adjusted to the concentration equivalent to 250 mg plant sample/ml. Flumethrin standard was prepared by diluting Bayticol (conc. 6 % w/v of flumethrin) with 70 % ethanol to a final concentration of 30 ng/ml.