The separation of the „Original‟ isolates from the “Duplicates‟ was done to dereplicate the isolates and avoid investigating the same strain more than once. A similar strategy was adopted by Watanabe et al. (2003) in order to group their actinomycete isolates based on cultural characteristics observed after cultivation on ISP#2 medium after one week. They based their dereplication on the presence of aerial mycelium, colour of the aerial mycelium, colour of the substrate mycelium and whether a soluble pigment was produced.
2.3.5