4. Conclusions
Next-generation sequencing was used to reveal microbial population
dynamics during spontaneous fermentation of Grenache
grapes. We described the persistence of Gluconobacter species
during alcoholic fermentation suggesting that the AAB and LAB
populations present during spontaneous wine fermentation are
more abundant than previously thought. Candida and Saccharomyces
imposed at the end of the fermentation and finished it successfully,
which is different of what a previous HTS study have
described for Chardonnay varietal. Additional work could be
necessary to analyze more grape varieties and compare the obtained
results. Our data suggest HTS techniques are useful for
assessing microbial changes in wine fermentations of different
grape varieties or under different treatments and this information
could be used by winemakers to drive fermentation processes and
to set up the most suitable environmental conditions to enhance
wine characteristics.
Acknowledgments
The present work was supported by Wildwine EU Project (grant
agreement 315065). M.C. Portillo is the recipient of a Beatriu de