Previously, Restriction enzyme-mediated integration (REMI)
transformed Trichoderma mutants have been reported for improving
degradation of cyanides (Zhou et al., 2007) and for improved
degradation of organophosphate pesticide, dichlorvos, in the soil
(Tang et al., 2009). Additionally, REMI technique has been utilized
to successfully construct Trichoderma transformants for growth
inhibition of an infectious pathogen, Ascosphaera apis, in honeybees
(data not shown). Thus, the objectives of this study were to generate
REMI transformed Trichoderma mutants and screening of transformants
for higher biodegradation ability against neonicotinoid
insecticide, imidacloprid.