it was centrifuged at 120,000 g for 15 min. A 1 mL aliquot of the resulting supernatant was filtered through a 0.22 μmmicropore filter and evaluated in a High Speed Amino Acid Analyzer (Model Hitachi L-8800).
it was centrifugedat 120,000 g for 15 min. A 1 mL aliquot of the resulting supernatantwas filtered through a 0.22 μmmicropore filter and evaluated in aHigh Speed Amino Acid Analyzer (Model Hitachi L-8800).