Newrapidmethodoflateral flowenzymeimmunoassay(LFEIA)forprogesteronedetectioninwhole
cows’ milk wasdeveloped.Thetestsystemutilizedhorseradishperoxidaseasalabelalongwiththe
substratesolutioncontaining3,3’,5,5’-tetramethylbenzidineanddextransulfatetoobtainaninsoluble
blue coloredproductoftheenzymereactiononasurfaceofanalyticalmembrane(testandcontrollines).
SeveralaspectsofLFEIAwereoptimized:timeofthesignaldetection,membranematerialsandassay
conditions. ResultingcompetitiveLFEIAcanbeperformedwithin15minuteswiththelimitof
progesteronedetectionof0.8ng/ml.Progesteroneconcentrationinwholemilksampleswasdetermined
by LFEIAandenzyme-linkedimmunosorbentassay(ELISA).Theresultsobtainedwereingood
correlation (R¼0.97,n¼46). ThusnewsensitiveLFEIAcanbesuccessfullyusedforon-sitemonitoring
of oestrusstatusofcows’ reproductivesystemandforearlynone-pregnancydetection.Themethodis
fast, easytoperformandneedsnopreliminarysamplepreparation.
Newrapidmethodoflateral flowenzymeimmunoassay(LFEIA)forprogesteronedetectioninwholecows’ milk wasdeveloped.Thetestsystemutilizedhorseradishperoxidaseasalabelalongwiththesubstratesolutioncontaining3,3’,5,5’-tetramethylbenzidineanddextransulfatetoobtainaninsolubleblue coloredproductoftheenzymereactiononasurfaceofanalyticalmembrane(testandcontrollines).SeveralaspectsofLFEIAwereoptimized:timeofthesignaldetection,membranematerialsandassayconditions. ResultingcompetitiveLFEIAcanbeperformedwithin15minuteswiththelimitofprogesteronedetectionof0.8ng/ml.Progesteroneconcentrationinwholemilksampleswasdeterminedby LFEIAandenzyme-linkedimmunosorbentassay(ELISA).Theresultsobtainedwereingoodcorrelation (R¼0.97,n¼46). ThusnewsensitiveLFEIAcanbesuccessfullyusedforon-sitemonitoringof oestrusstatusofcows’ reproductivesystemandforearlynone-pregnancydetection.Themethodisfast, easytoperformandneedsnopreliminarysamplepreparation.
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