In HPLC analysis of catechins, different detection methods
have been tried. Several authors performedHPLC analysis of catechins
by using a conventional UV detector of varying wavelength.
It has been reported that an optimal detection sensitivity
for different catechins could be obtained with absorbance at 280
nm (31,52,54). Another study demonstrated a higher sensitivity
at UV 205 nm (55). Dalluge et al. (27), Bronner et al. (30), and
Mizukami et al. (53)mentioned the use of 210 nm, while Goto et
al. (25) used 231 nm. In the proposed study, the UV spectra of all
studied compounds were reviewed using DAD. The DAD acquisition
wavelength was set in the range 200–400 nm. The results
demonstrated that an higher sensitivity was obtained in the
range 205–210 nm with the use of acetonitrile instead of
methanol as the eluent. Under the described conditions (HPLC
apparatus and chromatographic conditions section), we
achieved best results at wavelength of 210 nm.