The pork patties were collected at the indicated sampling day.The surface (S) and the inner (I) parts were divided using a knife. The surface part was approximately 0.5 cm depth of the entire patty. The surface part and the inner part were weighted upon sampling and amounted approximately 45% and 55%, respectively,of the total meat patty.The surface and the inner part were employed for analysis immediately or following vacuum packing and storage at 80 C till analysis.