The selected strains (13) were tested for hemolytic activity
following protocol proposed by
Argyri et al. (2013)
. Bile salt hy-
drolase (BSH) activity was performed using MRS agar plates sup-
plemented with 0.5% (w/v) of taurodeoxycholic acid (TDCA, Sigma).
Overnight MRS broth cultures (15
m
l) were inoculated onto the agar
medium, which was then incubated anaerobically for 48 h at 37
C.
BSH activity was present when taurodeoxycholic acid precipitated
in the agar medium below and around a colony. The strain
L. acidophilus
DRU was used as positive control.