The polymorphism of A781G locus of the growth hormone (GN) gene was analyzed in 112 Vembur sheep. Haelll-RFLP of the GN gene (422 bp) existence of two genotype viz., AA (366 and 56 bp) and AB 422, 366 and 56 bp),and two alleles viz.,A and B.Genotypic frequencies for AA and AB were 0.71 and 0.29 for A and B respectively. BB genotype was absent.Highly significant Chi-square value (P