After a 24-h feed deprivation, 150 size-selected shrimp (with initial
wet bodyweight 1.21±0.01 g, mean±S.E.)were pooled into a large fiberglass
tank. Among the pooled shrimps, 30 were randomly sampled
for later analysis (including initial dry weight, amount of energy and
protein in shrimp). The remainder were randomly picked up, individually
weighed and stocked into 24 aquaria with each aquarium holding
five individuals. During the experiment, the shrimps were fed twice a
day (at 6 a.m. and 4 p.m.).