Fig. 2. Beads containing Acetobacter xylinum
Growth parameters of immobilized Acetobacter xylinum fermentation were observed in the decrease in pH and
the rate of formation of nata. The first fermentation time required to achieve a thickness of 0.8 cm was 11 days with
a pattern of decrease in pH as in Fig. 3. A decrease in pH up to day six and there is no longer a decrease up to day
11. After the first fermentation is over, then proceed with the second fermentation using immobilized cells of the
same. Even though the fermentation of immobilized Acetobacter xylinum a bit longer than its free cell, it shows that
the immobilized cell still can release microbial cellulose to form nata. The results showed that the pattern of the
second fermentation is not much different from the pattern of pH. After day 6 the pH remains stable until day 11.