Sample storage, processing and DNA isolation slightly differed
as samples from the involved localities were processed independently
in two laboratories. Selected samples of all five crayfish taxa
were analyzed at the Central Veterinary Institute in Lelystad, the
Netherlands (CVI). In parallel, other samples of four of these taxa
(all but P. leniusculus) and samples of E. sinensis were analyzed at
the Department of Ecology, Charles University in Prague, Czech
Republic (CUNI). To confirm correct detection of the pathogen
and to compare the quantitative results obtained in the two laboratories,
a selection of DNA isolates was analyzed both at CUNI and
at CVI.