Culture conditions: All the media for auxiliary bud induction and plantlet root
production contained 30 g·L
-1
sucrose as carbon source, 6.8 g·L
-1
agar. The pH of the
medium was adjusted to 5.8 before sterilizing in an autoclave at a temperature of 121
C
and at the pressure of 151 bs psi for 15 min. The cultures were kept at 25±1
C with 16 h
light cycle of a light intensity of 2500 lux in every 24 h. Each treatment comprising of 20
explants pieces was repeated twice.