Specifically, 35 mL of 2,20- azobis-2-amidinopropane (AAPH, 0.36 mol/L) was added and the
fluorescence was measured (ex ¼ 485 nm and em ¼ 535 nm) every minute using a Victor3 multilabel plate reader (PerkineElmer, Turku, Finland) maintained at 37 C until the lecture had declined to
less than 5% of the initial reading.