The cut-off titer with the strongest association with fetal loss was included in the multivariate model. A significant increased risk of fetal loss was found for animals seropositive to N. caninum (odds ratio (OR) = 3.36; 95% confidence interval (95% CI) = 1.27–8.89), Hardjo (OR = 1.84; 95% CI = 1.01–3.33), and Pomona in non-vaccinated cows (OR = 14.91, 95% CI = 1.73–128.84) at the ELISA titer ≥30, and MAT titers of ≥1:384 and ≥1:768 for a positive sample, respectively