The comparison between
unknown samples and previously
genotyped reference samples was
performed in relation to band
sharing within 2 different primer
combinations. It must be noted
that the possibility of using even
monomorphic bands (66–100%
per combination) allowed for
comparisons based on a limited
number of primer pairs. The
Genographer analysis of AFLPs
profiles among reference and
unknown samples allowed the correct graphical species identification and all different
species were correctly genotyped. Whenever the number of specimens from each
group was consistent with fine systematic classification and population considerations,
BSI and the percentage of polymorphic bands were calculated.