wheat gluten film formation:
the wheat gluten film was prepared be dissolving 9 g of gluten and 1.5 g of glycerol in 32.5 ml 98% + 67.5 ml of distilled water using a heating magnetic stirrer at 70 C and centrifuged at 300 rpm for 15 min. in room temperature. The pH solution was adjusted to pH 10 using ammonium hydroxide. in this trial,the described film formation solution mentioned above was modified by adding 1 ml of thyme essential oil or