the amount of DNA after the pre-PCR and the smallest and largest frag ment sizes of the resulting DNA; as well as the amount of final library and the smallest and largest fragment size of remaining QC was performed after the initial DNA-shearing and cleanup the and clean-up (Qcz, from the final library after post-PCR and clean-up (QC3). Statistical analysis To examine the correlation between parameters, Pearson, Spearman, and Kendall correlation coefficients were com- puted for each pair of parameters. U-test [10] was