2.2. Ethylene and CO2 production
Ethylene and CO2 production was determined at 0, 7 and 14
days of storage. Twelve fruits were analyzed for each time,
temperature and treatment, i.e. 4 replicates of 3 fruits each. Once
removed from storage chambers, fruit was enclosed in sealed 10-L
containers for 6 h at 4 C or 20 C, depending on the experiment.
After this incubation period, gas samples were taken and ethylene
content was determined three times by gas chromatography in a
Varian 3900 GC fitted with a flame ionization detector (FID). In the
same way, CO2 was measured three times with headspace
analyzers Check mate II (Dansensor). Ethylene production and
respiration rate were expressed as nL g1 6 h1 and mg kg1 6 h1
,
respectively.
2.