The crude toxin extracts (from the ovaries of L.sceleratus puffer fish (4.5, 9.0, and 18.0 g crude extract/100 g paint) and only one concentration(3.0 g crude extract/100 g paint) from the skin mucus of A. hypselogenion puffer fish were mixed)
with an inert simple paint formulation (consists of (vinyl chloride–vinyl acetate) copolymer using tricresyl phosphate as plasticizer.