Products were validated on 1% agarose and diluted 10-fold to use as templates of subsequent selective amplification.
6 primer combinations, including E-AGA/MCAC, E-ACC/M- CAT, E-AAC/M-CAG, E-AAC/M-CTT, E-AGC/M-CAG and E-AGG/M-CTA, were chosen to conduct selective amplification for generating high quality bands in preliminary experiments.