The TMA of the crude mangosteen extract was determined usingthe pH differential method (Giusti and Wrolstad, 2005). A singlebeam UV–vis spectrophotometer (DR2800, Hach, USA) was usedfor spectral measurements at wavelength at 510 and 700 nm with resultsare expressed as mean value ± standard deviation (n = 6) and sta-tistically analyzed using Student-t’s distribution and for significantdifference if p < 0.05.