This study aimed to (1) develop and validate a multiresidue method for determination and quantification of ciprofloxacin
(CIP),
difloxacin (DIFL),
enrofloxacin (ENR),
norfloxacin (NOR),
sarafloxacin (SARA), and
oxytetracycline (OXY)
in aquaculture waters and surrounding water bodies and (2) provide the first Portuguese data to utilize in assessment of risk of adverse effects.