Fermentation is a commercialized process to produce bioethanol especially in Brazil and the United State. Carbohydrate content of microalgae especially starch or cellulose can be targeted for bioethanol production through biochemical conversion, using yeast to carry out alcoholic fermentation (Brennan and Owende, 2010). Due to its high tolerance to bioethanol concentration, Saccharomyces cerevisiae is widely used as the fermentation microbe (Balat et al., 2008). However, this kind of yeast consumes only simple sugar which is glucose. Therefore, starchy materials need to be converted to simple sugar through acid, alkaline or enzymatic hydrolysis. Enzymatic hydrolysis is an efficient method since it does not produce any fermentation inhibitors and extreme conditions is not required (Choi et al., 2010). Current improvement in enzyme processing technologies could reduce the cost issue for enzymatic hydrolysis in near future.