in the concentration. According to various authors, when PHA
content in the cytoplasm is high or when operational conditions
are unfavorable for the cell growth, i.e. high temperatures,
strong shear stress, or competition for space and nutrients, the
cell can continuously synthesize and release proteins to the
media, increasing the presence of amino groups ( NH2) in the
fermented broth (Bleves et al., 2010). Pseudomona bacteria
have showed that behavior. Amino compounds in proteins form
colored complexes with indophenol same as ammonia (NH4
+)