diluted hormone,17α-OHP and 17α-OHPL were injected(with 27-gauge needle mounted onto 1 ml syringe)at the third walk ingleg of each crab. This step followed standardized procedures of Reddy et al. (2006) and Fatihah et al. (2014). Each crab was injected either, with concentrations of 0.01 μg/g BW or 0.1 μg/g BW. Then, evaluations were carried out at intervals of 10 days and covered hormone level