Shoot cultures of C. glabra (USNA clone NA65636) were propagated
in vitro on shoot proliferation media consisting of full
strength Linsmaier and Skoog (LS) media (Linsmaier and Skoog,
1965) supplemented with 1.8 M 6-benzyl adenine (BA) and
0.5 M indole-3-butyric acid (IBA), and solidified with 0.15% (w/v)
GelriteTM (Caisson Laboratories Inc., North Logan, UT), and 0.35%
(w/v) agar (Sigma® St. Louis, MO). Nine plantlets with 4 or 5
internodes were cultured per Magenta® GA7 vessel. Shoot cultures
were grown in a controlled environment growth room
with continuous light from cool-white fluorescent lights (Ecolux®
F40T12 bulbs) and a photosynthetically active radiation (PAR) of
200–300 mol m−2 s−1 at 23
±
1 ◦C. Shoot cultures were subcultured
to new media at 4–5 week intervals.