4.4. Effect of order of reagents addition
Order of the reagent addition was studied using optimized volumes
of the corresponding reagents through the recommended
procedure. The phosphate results obtained are shown in Table 4.
The results are indicating that the change in order of addition of
reagents is not affecting the absorbance values. However, for maintaining
the uniformity of the order of reagents addition, Series No. 1
of Table 4 was followed throughout the course of the determination
of phosphate.