Freshly air-dried fins and scales of
Geophagus brasiliensis were also processed for comparison.
Ethanol-fixed tissues were then dried on a
filter paper, cut into small pieces and placed in 4 ml
of a TNES-digestion buffer (10 mM Tris-HCl pH 8.0;
125 mM NaCl; 10 mM EDTA pH 8.0; 0.5 %SDS;
4M urea) inside a 15 ml tube.