A subset of liver tissues was fixed in Trump’s fixative followed by fixation in
1% osmium tetraoxide for 1 hour. Tissues were subsequently dehydrated in a graded ethanol series, infiltrated with epoxy propylene oxide, and embedded in epoxy resin. The epoxy block was then sectioned with a Leica EM UC6 ultramicrotome, stained with uranyl acetate followed by lead citrate, and examined with a JEM-1400 transmission electron microscope equipped and confirmation of alveolate spores.