Plants are associated with a wide range of microorganisms throughout their life cycle, and some interactions
result on plant benefits. Trichoderma species are plant beneficial fungi that enhance plant
growth and development, contribute to plant nutrition and induce defense responses. Nevertheless, the
molecules involved in these beneficial effects still need to be identify. Polyamines are ubiquitous molecules
implicated in plant growth and development, and in the establishment of plant microbe interactions.
In this study, we assessed the polyamine profile in Arabidopsis plants during the interaction
with Trichoderma virens and Trichoderma atroviride, using a system that allows direct plant-fungal contact
or avoids their physical interaction (split system). The plantlets that grew in the split system exhibited
higher biomass than the ones in direct contact with Trichoderma species. After 3 days of interaction, a
significant decrease in Arabidopsis polyamine levels was observed in both systems (direct contact and
split). After 5 days of interaction polyamine levels were increased. The highest levels were observed with
T. atroviride (split system), and with T. virens (direct contact). The expression levels of Arabidopsis ADC1
and ADC2 genes during the interaction with the fungi were also assessed. We observed a time dependent
regulation of ADC1 and ADC2 genes, which correlates with polyamine levels. Our data show an evident
change in polyamine profile during Arabidopsis e Trichoderma interaction, accompanied by evident
alterations in plant root architecture. Polyamines could be involved in the changes undergone by plant
during the interaction with this beneficial fungus.
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Plants are associated with a wide range of microorganisms throughout their life cycle, and some interactionsresult on plant benefits. Trichoderma species are plant beneficial fungi that enhance plantgrowth and development, contribute to plant nutrition and induce defense responses. Nevertheless, themolecules involved in these beneficial effects still need to be identify. Polyamines are ubiquitous moleculesimplicated in plant growth and development, and in the establishment of plant microbe interactions.In this study, we assessed the polyamine profile in Arabidopsis plants during the interactionwith Trichoderma virens and Trichoderma atroviride, using a system that allows direct plant-fungal contactor avoids their physical interaction (split system). The plantlets that grew in the split system exhibitedhigher biomass than the ones in direct contact with Trichoderma species. After 3 days of interaction, asignificant decrease in Arabidopsis polyamine levels was observed in both systems (direct contact andsplit). After 5 days of interaction polyamine levels were increased. The highest levels were observed withT. atroviride (split system), and with T. virens (direct contact). The expression levels of Arabidopsis ADC1and ADC2 genes during the interaction with the fungi were also assessed. We observed a time dependentregulation of ADC1 and ADC2 genes, which correlates with polyamine levels. Our data show an evidentchange in polyamine profile during Arabidopsis e Trichoderma interaction, accompanied by evidentalterations in plant root architecture. Polyamines could be involved in the changes undergone by plantduring the interaction with this beneficial fungus.©
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