There is a considerable interest to optimize the carbon sources for lipase production and to try cheaper substrates alternatives . It was reported that it is difficult to get all required features from a single substrate; however it could be achieved by combination of different substrates . Accordingly, to enhance the growth and production of lipase from Candida rugosa ATCC 14830, a novel route has been tested. In the present work, a novel fermentation media using a combination of different substrates like fatty acids residues, soapstock, and olive oil were chosen for lipase production. In this way, a sustainable way of hydrolysis could be provided with keeping an economically feasible rout of production of fatty acids.