Segregating populations were managed by pedigree selection appropriate for self-pollinating crops (Fehr, 1987). Sixteen protocols to assess disease resistance, horticultural and fruit traits (Tables 1 and 2) were used to screen populations and lines. Selection and generation advance began in 2008 with a segregating triple-cross F7 population (Table 3) and continued until completion of F1 lines in June 2012. Selection was based on individual plant performance
in the F1 and F3 generations and single plot progeny rows (30 plants per plot) with individual plant selection practiced within rows from the F7 to F2 generations. Two tomato crops were produced in southern Taiwan during the dry season (October–February and March–June) allowing two cycles of generation advance per year. Seed was sown in trays and seedlings were maintained in a
plastic house for about 30 days before field transplanting.