The cartridge was conditioned with two volumes (10 mL) of ultrapure water prior to the application of the concentrated extracts. Compounds were sequentially eluted with 1 volume (5 mL) of increasing concentrations of methanol:water as follows: 0:100, 20:80, 50:50, 80:20, 100:0 (v/v). Fractions were individually collected and tested for antifungal activity as described below.