Membrane separation efficiency was determined using permeations
of various protein solutions. BSA, cellulase and trypsin solutions
were employed in the test, corresponding to different
molecular weights of 66 kDa, 45 kDa and 23.8 kDa (atomic mass
unit, 1000 Dalton), respectively. The protein concentration was
determined by a UV/Vis spectroscope and a standard curve was
prepared prior to sample analysis. The separation efficiency was
determined by comparing the concentration of penetrated protein
solution to feeding solution.