solidified MS medium without plant
growth regulators in the presence of 1 mg/l silver nitrate and 0.05% activated
charcoal. After 4 weeks of being cultured those shoots were ready for root
induction (Fig. 1g); elongated single shoots, 2–3 cm long were excised from the
multiple shoots and transferred to solidified half-strength MS medium
supplemented with 5 mg/l IBA, 1 mg/l silver nitrate, 3% sucrose and 0.05%
activated charcoal. The shoots developed roots within 3 weeks of being
transferred and there was a 100% rate of root induction (Fig. 1h).