2.2.7. Extraction of lignans from sesame seedsLignans from the sesame seeds were extracted following theprotocol of Wang et al. (2012). 100 mg of sesame seeds of each ofthe 12 selected varieties were homogenized in pestle and mortaralong with 5 ml of 80% ethanol. The extract was transferred into30 ml Oakridge tubes and centrifuged for 10 min at 8000 rpm inSigma 6K15 centrifuges, set at room temperature. The supernatantwas transferred to a fresh Oakridge tube and the extraction wasrepeated. Ethanol was evaporated using rotary evaporator. To thedry residue, 1 ml of 80% methanol was added and filtered through0.45 m nylon membrane before HPLC analysis.