The effect of hyperbaric treatments on major hydrophilic and lipophilic antioxidants and antioxidantactivity in tomato fruit, using ORAC and TEAC assays, was studied. Early breaker stage greenhouse growntomatoes were subjected to different pressure and temperature conditions, including 0.1 (ambient atmo-spheric pressure, control), 0.3, 0.5, 0.7 and 0.9 MPa at 20◦C, and 0.1 MPa at 13◦C (cold treatment) for 4days, followed by ripening at 20◦C for 5 and 10 days. Hyperbaric treatment significantly affected lycopenecontent by inhibiting, then enhancing its accumulation during treatment and ripening, respectively.In general, ascorbic acid and total phenolic contents increased as time progressed but generally werenot affected by hyperbaric pressure treatment. All antioxidants were found in lower concentrations intomatoes treated at 13◦C. The trend in antioxidant activity obtained from both ORAC and TEAC assayswas generally similar. No significant effect of hyperbaric treatment on lipophilic antioxidant (LAA) andhydrophilic antioxidant (HAA) was observed compared with control tomatoes at 13 and 20◦C. However,the ORAC assay showed that hyperbaric treated tomatoes had significantly higher HAA than 13◦C treatedtomatoes. Overall, hyperbaric treatment at 20◦C has potential to extend tomato shelf-life during shorttreatment durations without adverse impact on quality during ripening.