COD = 34 ± 0.1 mg.L−1; N-NH4
+ = 22.6 ± 0.1 mg.L−1;
N-NO3
− = 2.2 ± 0.1 mg.L−1 and P = 3.9 ± 0.1 mg.L−1.
The compounds employedwere analyzed byHPLC(Varian)
equipped with aUV-vis PDAdetector model 230 under
the following conditions: column, Mediterranea C18 250×
4.6mmi.d.; an isocratic mobile phase: 40% acetonitrile and
60% H2O (0.6 mL.min−1) with single compounds and 70%
acetonitrile and 30% H2O (0.8 mL.min−1) with two compounds.