PCR products digested with Mbo II restriction enzyme. Lanes 1, 3, 5, 7 show the PCR products before the effect of restriction enzyme (583-neucleotide band is visible) and Even columns are related to PCR products after the enzymatic diges-tion. Lanes 4, 6 show samples without Mediterranean mutation. Lane 2 shows female heterozygote with Mediterranean muta-tion. Lane 8 shows a male with Mediterranean mutation