Additional dissociation-curve analysis was performed and showed a single melting curve in all cases. The relative expression in each sample was calculated according to the method described by Pfaffl (Pfaffl, 2001).
Additional dissociation-curve analysis wasperformed and showed a single melting curve in all cases. The relativeexpression in each sample was calculated according to the method describedby Pfaffl (Pfaffl, 2001).