Drug treatments and FM4-64 staining
Growing root apices were exposed to the following
drugs: 2,3-butanedione monoxime (10 mM), latrunculin
B(1mM forA. thalianaand 10mM forM. truncatula),
jasplakinolide (1mM/5mM), brefeldin A(10mM for
A. thalianaand 100mM forM. truncatula), wortmannin
(10mM), and oryzalin (1mM). The drugs were diluted in
culture medium and directly added to the roots of
transgenic M. truncatulaor A. thaliana. Regarding
FM4-64, stock solution (1 mg/ml) was prepared in
DMSO and used at 17.5mM forM. truncatula. Before
FM4-64 treatment, plants were incubated for 25 min at
61C to slow down endocytosis.
Drug treatments and FM4-64 stainingGrowing root apices were exposed to the followingdrugs: 2,3-butanedione monoxime (10 mM), latrunculinB(1mM forA. thalianaand 10mM forM. truncatula),jasplakinolide (1mM/5mM), brefeldin A(10mM forA. thalianaand 100mM forM. truncatula), wortmannin(10mM), and oryzalin (1mM). The drugs were diluted inculture medium and directly added to the roots oftransgenic M. truncatulaor A. thaliana. RegardingFM4-64, stock solution (1 mg/ml) was prepared inDMSO and used at 17.5mM forM. truncatula. BeforeFM4-64 treatment, plants were incubated for 25 min at61C to slow down endocytosis.
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