The monosaccharides of the dried mycelia were measured as described earlier.
The liberated monosaccharides were reduced with sodium borohydride followed by conversion to
alditols by addition of 0.1 N HCl in methanol.
After drying, residues were suspended in water and extracted with freshly distilled chloroform.
Identification and estimation of neutral and amino monosaccharides as their alditol acetates were performed by Hewlett-Packard 5890 Gas-Liquid Chromatograph using SP 2340 and HP-5 columns with nitrogen as carrier gas.
The monosaccharide distribution is expressed as percent of total sugars present.