respectively. Such specific uptake rates for thiosulfate and glucose can be attributed to Pseudomonas spp. possessing an extensive biochemical system. The results also indicate that sulfate concentrations increased slowly during the experiment periods, except on the 8th and 9th days. The average conversion rate of thiosulfate during the initial 7 days was 15%, whereas the conversion ratios for the 8th and 9th days were 50%. Because microorganism autolysis can liberate intracellular sulfate, the dramatic increase in sulfate concentration from 15% to 50% may be due to dead P. putida CH11, which can be justified by the decay in cell numbers. The 15% conversion ratio implies that P. putida CH11 only oxidized a small amount of thiosulfate to sulfate; therefore, a stable and neutral pH value was observed in the growth medium.