Saccharification of substrates by Aspergillus niger: For saccharification of substrates locally isolated fungal
culture Aspergillus niger was employed. The chemically treated substrates were autoclaved and inoculated with
sporulating mycelial mat of Aspergillus niger. Saccharification was carried out in stationary and shaking
methods for a period of six days at 300C and the process was monitored every 24 hrs for total sugars released
(24). For the shaking method an orbital shaking incubator was employed and shaking was performed at 100 rpm
at 300C temperature.